LNK174PETR enables the rapid conjugation of a pre-prepared lyophilized mixture containing R-Phycoerythrin (RPE)-Texas Red® label to an antibody or protein. Activation of proprietary reagents within the antibody-label solution results in directional covalent bonding of RPE-Texas Red® to the antibody.
The LYNX Rapid Conjugation kit®can be used to label small quantities of antibody/protein at near neutral pH, allowing a high conjugation efficiency with 100% antibody recovery.
Click herefor further information on our LYNX Rapid Conjugation kits®.
The following buffer solutions are recommended:for preparing the antibody:
10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.
Do not use buffers containing nucleophilic components e.g primary amines and thiols since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA, up to 50% Glycerol and common non-buffering salts and sugars have little or no effect on conjugation efficiency.
Due to the large size of the RPE-Texas Red® label the quantity of RPE--Texas Red® dye is in slight molar excess. Approximately 10ug of IgG will give a 1:1 molar ratio of antibody:. For optimal results the antibody volume should be around 4ul-10ul.
Storage
Store kit at -20oC only.
Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing.
Shelf Life
12 months after despatch.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Conjugation
We recommend that for each conjugation the user determines the best antibody:conjugate ratio.
Instructions For Use
1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.
2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.
3. Replace cap onto vial and incubate in the dark at room temperature (20-25oC) for 3 hours, or overnight if preferred.
4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.
AbD Serotec LYNX Rapid Conjugation kits®are offered for research purposes alone, and are not intended for human, therapeutic or diagnostic use. The purchase of this conjugation kit conveys to the buyer (whether the buyer is a not-for-profit, academic or for-profit entity) the non-transferable right to use the amount of product purchased and the components of the product for in-house research. The buyer shall not sell or otherwise transfer this product, its components, or materials prepared therefrom to any third party. The buyer shall not use this product or its components for commercial purposes. For the avoidance of doubt, ‘commercial purposes’ means any activity by a party for consideration and includes, without limitation, use of the product or its components (i) in the manufacturing of conjugated materials (e.g. labeled antibodies), (ii) to provide a service, information or data, (iii) for therapeutic, diagnostic or prophylactic purposes, or (iv) for repackaging/resale, whether or not such product or its components are resold for use in research. The use of this product by the buyer constitutes agreement with the terms of this limited use label license for LYNX products.
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