LNK152CY3 enables the rapid conjugation of a pre-prepared lyophilized mixture containing Cyanine Dye3 (Cy3) label to an antibody or protein. Activation of proprietary reagents within the antibody-label solution results in the coupling of Cy3 to the antibody.
The LYNXTMkit can be used to label small quantities of antibody/protein at near neutral pH, allowing a high conjugation efficiency with 100% antibody recovery.
Click herefor further information on our LYNXTMRapid Conjugation kits.
Click herefor frequently asked questions about our LYNXTMRapid Conjugation kits.
The following buffer solutions are recommended:for preparing the antibody:
10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.
Do not use buffers containing nucleophilic components e.g primary amines and thiols since they may react with LYNXTMchemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.
It is recommended that 1-2mg antibody be used in each labelling reaction. For optimal results the antibody volume should be 400-1000ul, at a concentration range of 1-4mg/ml.
Storage
Store kit at -20oC only.
Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing.
Shelf Life
12 months after despatch.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Conjugation
We recommend that for each conjugation the user determines the best antibody:conjugate ratio.
Instructions For Use
1.To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.
2.Pipette the mixed antibody-modifier sample directly onto the LYNXTMlyophilized mix and gently pipette up and down twice to resuspend.
3.Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.
4.After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Licensed Use
AbD Serotec LYNXTMconjugation kits are offered for research purposes alone, and are not intended for human, therapeutic or diagnostic use. The purchase of this conjugation kit conveys to the buyer (whether the buyer is a not-for-profit, academic or for-profit entity) the non-transferable right to use the amount of product purchased and the components of the product for in-house research. The buyer shall not sell or otherwise transfer this product, its components, or materials prepared therefrom to any third party. The buyer shall not use this product or its components for commercial purposes. For the avoidance of doubt, ‘commercial purposes’ means any activity by a party for consideration and includes, without limitation, use of the product or its components (i) in the manufacturing of conjugated materials (e.g. labeled antibodies), (ii) to provide a service, information or data, (iii) for therapeutic, diagnostic or prophylactic purposes, or (iv) for repackaging/resale, whether or not such product or its components are resold for use in research. The use of this product by the buyer constitutes agreement with the terms of this limited use label license for LYNXTMproducts.
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