Intended Use
LNK006P enables the rapid conjugation of a pre-prepared lyophilized mixture containing Horseradish peroxidase (HRP) label to an antibody or protein. Activation of proprietary reagents within the antibody-label solution results in directional covalent bonding of HRP to the antibody.
The LYNX Rapid Conjugation kit
® can be used to label small quantities of antibody/protein at near neutral pH, allowing a high conjugation efficiency with 100% antibody recovery.
Click here for further information on our LYNX Rapid Conjugation kits
®.
Click here for frequently asked questions about our LYNX Rapid Conjugation kits
®.
Reagents In The Kit
3 Vials of 10ug LYNX lyophilized HRP mix
1 Vial LYNX Modifier reagent
1 Vial LYNX Quencher reagent
Preparing The Antibody
The following buffer solutions are recommended for preparing the antibody:
10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.
Do not use buffers containing nucleophilic components e.g primary amines and thiols since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.
Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.
The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 10µg HRP you need to add between 10-40µg of antibody. For optimal results the antibody volume should be up to 10ul, at a concentration range of 0.5-5.0mg/ml.
Storage
Store kit at -20oC only.
Newly-conjugated antibody can be stored at 4oC. For long term storage however, the addition of a preservative is recommended, although sodium azide should be avoided.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing.
Shelf Life
12 months after despatch
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit
www.abdserotec.com/protocols.
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Application Name |
Yes |
No |
Not Determined |
Suggested Dilution |
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Conjugation |
|
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We recommend that for each conjugation the user determines the best antibody:conjugate ratio.
Instructions For Use
1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.
2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.
3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.
4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use.
MSDS
Health And Safety Information
Licensed Use
These products and the methodology of conjugation are patent protected under United Kingdom patent number 2446088 and associated international patent applications. The purchase of this product conveys to the buyer the limited, non exclusive non-transferable right (without the right to resell repackage or further sublicense) under these patents to use the product to make conjugates for research and development purposes only. The purchaser cannot sell or otherwise transfer this product, or its components, or materials or data made using this product, or its components to a third party. Further information on purchasing licenses for diagnostic and other uses may be obtained by contacting AbD Serotec, at. Endeavour House, Langford Business Park, Langford Lane, Kidlington, Oxon. OX5 1GE UNITED KINGDOM. Tel: +44 1865 852 700. E-mail:
sales@abdserotec.com
Regulatory
For research purposes only
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