The Intracellular Protein Buffer Reagent Kit is comprised of the reagents that are intended for use with Human, Mouse and Rat Intracellular Singleplex Bead Kits for in vitro quantitative determination of analytes in cell lysates and tissue homogenates. Singleplex Bead Kits have been developed to maximize flexibility in experimental design, permitting the measurement of one or multiple analytes in panels designed by the researcher. These reagents are suitable for use with the Luminex® 100™/200™ and the FLEXMAP 3D® systems.
• Superior performance—accurate, reproducible, and sensitive quantitation of multiple proteins • High quality—fully qualified antibodies permit excellent specificity and sensitivity • Fast and easy protocols—perform your assay and analyze your data typically in less than one day
Luminex® xMAP® Technology—Effective and Proven Analytical Tool The Human, Mouse and Rat Intracellular Singleplex Bead Kits used in conjunction with the Intracellular Protein Buffer Reagent Kit are based on xMAP® technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex® assays. Polystyrene microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead is given a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay, utilizing a 96-well microplate format. At the completion of the sandwich immunoassay, beads can be read using theLuminex® 100™/200™ or FLEXMAP 3D® detection systems, instruments based on flow cytometry, two lasers, real-time digital signal processing, and xPONENT® software to distinguish bead color (analyte) and assay signal strength (PE) fluorescence intensity.
More Validation for Confidence in Your Results The Human, Mouse and Rat Intracellular Singleplex Bead Kits that are used with the Intracellular Protein Buffer Reagent Kit have been validated using the same rigorous criterion that distinguishes Luminex® assays from other commercially available assays. With more than 20 years of experience, our fully qualified antibodies help ensure the specificity and sensitivity of our assays. The Intracellular Protein Buffer Reagent Kit comes with the assay protocol to be followed in conjunction with the Human, Mouse and Rat Intracellular Singleplex Bead Kits chosen (See Documents section below).
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For Research Use Only. Not for use in diagnostic procedures.
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