Novex® Zymogram Gels are excellent tools for detecting and characterizing proteases that utilize casein or gelatin as a substrate. Proteases are run on a gel containing one of these substrates under denaturing conditions and visualized as clear bands against a dark background following a simple renaturing, developing, and staining protocol. Zymogram gels are commonly used to detect matrix metalloproteinases.
Formulation:All Novex® Zymogram Gels are Tris-Glycine gels with a substrate (gelatin or casein) incorporated into the gel. They are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, highly purified water and casein or gelatin as the substrate. All have a 4% stacking gel.
Zymogram Gel Types:The 10% Zymogram (Gelatin) Gel is a 10% Tris-Glycine gel with 0.1% gelatin as the substrate. The 12% Novex® Zymogram (Casein) Gel is a 12% Tris-Glycine gel with 0.05% casein as the substrate.
原厂资料：
Novex® Zymogram Gels are excellent tools for detecting and characterizing proteases that utilize casein or gelatin as a substrate. Proteases are run on a gel containing one of these substrates under denaturing conditions and visualized as clear bands against a dark background following a simple renaturing, developing, and staining protocol. Zymogram gels are commonly used to detect matrix metalloproteinases.
Formulation:All Novex® Zymogram Gels are Tris-Glycine gels with a substrate (gelatin or casein) incorporated into the gel. They are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, highly purified water and casein or gelatin as the substrate. All have a 4% stacking gel.
Zymogram Gel Types:The 10% Zymogram (Gelatin) Gel is a 10% Tris-Glycine gel with 0.1% gelatin as the substrate. The 12% Novex® Zymogram (Casein) Gel is a 12% Tris-Glycine gel with 0.05% casein as the substrate.
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