Nitrocellulose Pre-Cut Blotting Membranes (0.45 µm pore size) are high-quality membranes ideal for blotting proteins and nucleic acids. The 0.45 µm pore size is ideal for the transfer of most proteins (>20 kDa) and nucleic acids (>300 bp). Invitrogen™ nitrocellulose membranes are:
• Composed of 100% pure nitrocellulose to provide high-quality transfer
• Contain no support fabrics or detergents
• Are compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, fluorescence, and radiolabeling
• Provide high-sensitivity with low background
• Are supplied in a pre-cut, pre-assembled membrane/filter paper sandwich for convenience
A protein's properties (i.e., charge, hydrophobicity, etc.) affect its ability to bind to membrane surfaces. Finding the right membrane may require experimenting with your specific protein on different membranes.
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