Novex® Tricine Gels are useful for resolving low molecular weight proteins and peptides. In the tricine system developed by Schaegger and von Jagow (1), tricine replaces glycine in the running buffer. This results in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.

Formulation: Novex® Tricine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. Novex® Tricine Gels have a 4% stacking gel and do not contain SDS. The tricine system requires SDS in sample and running buffers for best results.

Recommended Buffers: Use Tricine Running Buffers and Sample Buffers with Novex® Tricine Gels to obtain the benefits of this gel system. Novex® Tris-Glycine transfer buffer can be used for western blotting.