The LIVE/DEAD® Cell-Mediated Cytotoxicity Kit measures natural killer (NK) cell-mediated, lymphokine-activated killer (LAK) cell-mediated and T cell mediated cytotoxicity. Target cells are preincubated with the green-fluorescent membrane stain DiOC18 and then mixed with effector cells in the presence of the red-fluorescent, membrane-impermeant dye, propidium iodide. Live and dead target cells retain the green-fluorescent membrane stain; target and effector cells with compromised membranes exhibit red-fluorescent nucleic acid staining; live effector cells are nonfluorescent.
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For Research Use Only. Not for use in diagnostic procedures.
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