TnT® T7 Quick for PCR DNA is a rapid, convenient, coupled transcription/translation system designed for optimum protein expression from PCR templates. For most PCR templates, the TnT® T7 Quick for PCR DNA reactions produce up to 5 times more protein than other commercially available kits. The PCR-generated DNA can be used directly from the amplification reaction or purified by numerous commercially available kits and traditional methods.
Features - Benefits
Convenient: Directly from PCR, no cleanup necessary.
High Yield: Up to 5 times more expressed protein than standard translation reactions with linear templates.
Quick: One-tube reaction.
Complete: Reagents including Recombinant RNasin® Ribonuclease Inhibitor are included in the Quick Master Mix.
Good Value: One-tube format means no leftover reagents.
Reliable: The TnT® Systems are rigorously quality controlled to ensure the highest level of transcription/translation.
Applications
For more information, see the Protein Expression chapter of the Protocols & Applications Guide.
References
Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.