Rabbit Reticulocyte Lysate System, Nuclease Treated

产品编号：L4960 品牌：promega 原厂货号：L4960
产品分类：蛋白质表达与纯化 > 蛋白质表达系统 > 无细胞表达系统
应用分类：

包装：

30 reactions

运保温度:

–70°C

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其它组分：

Reticulocyte Lysate, Nuclease Treated【子货号：L416A，包装：5 x 200μl，，运保温度：–70°C 】

Amino Acid Mixture Minus Cysteine【子货号：L447C，包装：1 x 50μl，，运保温度：–70°C 】

Luciferase Control RNA【子货号：L456A，包装：1 x 10μl，，运保温度：–70°C 】

Amino Acid Mixture Minus Leucine【子货号：L995A，包装：1 x 50μl，，运保温度：–70°C 】

Amino Acid Mixture Minus Methionine【子货号：L996A，包装：1 x 50μl，，运保温度：–70°C 】

描述：

说明

Rabbit Reticulocyte Lysate Translation Systems, Nuclease Treated ( 经核酸酶处理的兔网织红细胞裂解物翻译系统) 可被用于鉴别mRNA 的种属，研究蛋白产物的特征，研究转录和翻译过程的控制机制。兔网织红细胞裂解物使用常规方法从新西兰白兔制得，保证每批的网织红细胞产量可靠而稳定。网织红细胞裂解后，用微球菌核酸酶处理提取物，以破坏内源mRNA，将本底的翻译反应减到最小。裂解液含有蛋白质合成所必需的细胞组分(tRNA，核糖体，氨基酸，起始、延伸和终止因子)。

特点

. 稳定：每一批产品的翻译产物可靠而稳定。

• 已经优化，直接可用：经过处理的兔网织红细胞裂解物已被优化，以达到最佳的翻译条件。该试剂盒中含有能量再生系统( 磷酸肌酸/ 磷酸肌酸激酶)，tRNA 混合物( 用以扩展可翻译的mRNA 范围)，氯高铁血红素( 防止对翻译的起始产生抑制作用)，以及氯化钾和醋酸镁。

• 可定制或批量订购：请访问www.promega.com/myway/，了解该产品的定制信息。

原厂资料：

Rabbit Reticulocyte Lysate Translation Systems are utilized in the identification of mRNA species, the characterization of their protein products and the investigation of transcriptional and translational control. Rabbit Reticulocyte Lysate is prepared from New Zealand white rabbits using a standard protocol that ensures reliable and consistent reticulocyte production in each lot. After the reticulocytes are lysed, the extract is treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum. The lysate contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors).

Features - Benefits

Consistent: Reliable and consistent translation with each lot.
Optimized and Ready to Use: The treated Rabbit Reticulocyte Lysate is optimized for translation and contains an energy-regenerating system (phosphocreatine/phosphocreatine kinase), a mixture of tRNAs (to expand the range of mRNAs that can be translated), hemin (to prevent inhibition of initiation), and potassium chloride and magnesium acetate.
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Applications

In vitro translation from mRNA, in vitro run-off transcripts or total RNA into protein.
Characterization of mRNA products.
Co-translational processing of secreted proteins by the addition of microsomal membranes to the translation reaction.

For more information, see the Protein Expression chapter of the Protocols & Applications Guide.

References

Pelham, H.R.B. and Jackson, R.J. (1976) Eur. J. Biochem. 67, 247–56.
Glass, C.A. and Pollard, K.M. (1990) Promega Notes 26, 6–9.