TNT? SP6 Coupled Reticulocyte Lysate System

• 产品编号：L4600      品牌：promega       原厂货号：L4600
• 产品分类：蛋白质表达与纯化 > 蛋白质表达系统 > 无细胞表达系统
• 应用分类：蛋白质研究 > 蛋白质表达 > 无细胞表达 > 原核无细胞表达系统

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The TnT® Coupled Reticulocyte Lysate Systems offer researchers an alternative for eukaryotic cell-free protein expression: a single-tube, coupled transcription/translation system. The TnT® Lysate Systems greatly simplify the process and reduce the time required to obtain in vitro translation results. Standard rabbit reticulocyte lysate translations commonly use RNA synthesized in vitro from SP6, T3 or T7 RNA polymerase promoters and require three separate reactions with several steps between each reaction. The TnT® Systems bypass many of these steps by incorporating transcription directly in the translation mix.

For optimal protein expression using the TnT® SP6 RNA polymerase, we recommend titrating magnesium acetate in 0.1mM increments between 0.1mM and 0.5mM. In some instances the addition of 0.2mM magnesium acetate has been shown to increase protein expression by 40%. Magnesium acetate is supplied only with Cat.# L4600 and L4601.

Features - Benefits

Use in Multiple Applications: The TnT® Systems are widely used for protein:protein interaction, protein:nucleic acid interactions, and more.
Save Time: Using a one-tube reaction, proteins are generated in one hour, not days, as with in vivo methods.
Complete System: All the reagents you need are provided (except radioisotopes).
Reliable: Eliminate solubility issues by using an in vitro mammalian system.
Dependability You Can Count On: The TnT® Systems are rigorously quality controlled to ensure the highest level of performance.

Applications

GST pull-downs (view an animation).
Gel shift assays (view an animation).
Co-immunoprecipitation (view an animation).
Characterization of protein modifications (view an animation).
Protein:RNA interactions.
Protein activity studies.
Confirmation of gene products.
Protein Truncation Test (PTT).
For more information about cell-free protein expression, see the Protein Expression chapter of the Protocols & Applications Guide.

References

Pelham, H.R.B. and Jackson, R.J. (1976) Eur. J. Biochem. 67, 247–56.
Krieg, P.A. and Melton, D.A. (1984) Nucl. Acids Res. 12, 7057–70.

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