The Flexi® Rabbit Reticulocyte Lysate System allows translation reactions to be optimized for a wide range of parameters, including Mg2+and K+concentrations and the choice of adding DTT. To help optimize Mg2+for a specific message, the endogenous Mg2+concentration of each lysate batch is stated in the product information included with this product. The Flexi® System also offers the choice of three amino acid mixtures and includes a control RNA encoding the firefly luciferase gene.
Features - Benefits
Improved Efficiency:In an optimized system, the quantity of protein produced can be increased as much as fourfold over that of a standard lysate reaction.
Easy Optimization:To aid in optimizing magnesium concentrations, the endogenous magnesium concentration is provided for each lot of Flexi® Lysate.
Choice:The Flexi® System contains three Amino Acid Mixtures, which enable different choices of radioisotopes.
Control Included:Luciferase Control RNA and Luciferase Assay Reagent are included with the system as a functional control. Only full-length luciferase is active.
Applications
Optimizing translation of low-expressing messages.
Oxidizing environment can be adjusted, which may be important for maintaining functional activity related to disulfide bridges.
Luciferase activity can be used for the detection of inhibitory contaminants in RNA preparations.
Investigation of translational control.
Screening of chemical libraries for effect on translation.
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