Rabbit Reticulocyte Lysate/Wheat Germ Extract Combination System

产品编号：L4330 品牌：promega 原厂货号：L4330
产品分类：蛋白质表达与纯化 > 蛋白质表达系统 > 无细胞表达系统
应用分类：

包装：

24 reactions

运保温度:

–70°C

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其它组分：

Reticulocyte Lysate, Nuclease Treated【子货号：L416A，包装：2 x 200μl，，运保温度：–70°C】

Wheat Germ Extract【子货号：L418A，包装：2 x 200μl，，运保温度：–70°C】

Potassium Acetate【子货号：L420A，包装：1 x 0.5ml，，运保温度：–70°C】

Amino Acid Mixture Minus Cysteine【子货号：L447A，包装：1 x 175μl，，运保温度：–70°C】

Luciferase Control RNA【子货号：L456A，包装：1 x 10μl，，运保温度：–70°C】

Amino Acid Mixture Minus Leucine【子货号：L995B，包装：1 x 175μl，，运保温度：–70°C】

Amino Acid Mixture Minus Methionine【子货号：L996B，包装：1 x 175μl，，运保温度：–70°C】

描述：

说明

Rabbit Reticulocyte Lysate/Wheat Germ Extract Combination System( 兔网织红细胞裂解物/ 麦胚提取物组合系统) 包含Rabbit Reticulocyte Lysate( 兔网织红细胞裂解液) 和Wheat Germ Extract( 小麦胚芽提取物)，以便对这两个系统的体外翻译进行比较。兔网织红细胞裂解液从新西兰白兔制得。小麦胚芽提取物是在提取物缓冲液中将小麦胚芽碾碎，再离心除去细胞碎片制得。两个系统都含有蛋白质合成所必需的细胞组分。系统已经过微球菌核酸酶处理，破坏了内源mRNA，使本底的翻译水平减到最低。

特点

. 可选择：可通过测试兔网织红细胞裂解物和小麦胚芽系统的性能，从而找到最佳的翻译系统。

• 稳定性：严格的质量控制保证批次之间的差异最小。

• 最佳表达：提供了醋酸钾以促进小麦胚芽提取物系统适用于范围广泛的mRNA。

原厂资料：

The Rabbit Reticulocyte Lysate/Wheat Germ Extract Combination System contains both Rabbit Reticulocyte Lysate and Wheat Germ Extract for comparing in vitro translation systems. Reticulocyte Lysate is prepared from New Zealand white rabbits. The Wheat Germ Extract is prepared by grinding wheat germ in an extraction buffer followed by centrifugation to remove cellular debris. Both systems contain the cellular components necessary for protein synthesis. The systems have been treated with micrococcal nuclease, which destroys endogenous mRNA and results in minimal background translation.

Features - Benefits

Choice: Test both Rabbit Reticulocyte Lysate and Wheat Germ Systems to find optimal translation systems.
Consistent: Rigorous quality control ensures minimal lot-to-lot variability.
Optimal Expression: Potassium Acetate is provided to enhance the Wheat Germ Extract System for a wide range of mRNAs.

Applications

For more information, see the Protein Expression chapter of the Protocols & Applications Guide.

Notes

Each system contains 2 × 200μl of both Rabbit Reticulocyte Lysate and Wheat Germ Extract; this is sufficient for 12 reactions using the Rabbit Reticulocyte Lysate and 12 reactions using the Wheat Germ Extract.

References

Pelham, H.R.B. and Jackson, R.J. (1976) Eur. J. Biochem. 67, 247–56.