The TnT® SP6 High-Yield Wheat Germ Protein Expression System, based on an optimized wheat germ extract, is a single-tube, coupled transcription/translation system designed to express proteins in only two hours. Protein synthesized, in the range of 10–100μg/ml, can be used in multiple proteomic-based applications, as well as in high-throughput analysis.
All components necessary for transcription/translation are provided in the extract, with the exception of the plasmid DNA or PCR template. Optional protein-labeling reagents must also be supplied by the user.
For custom wheat germ extract (depleted amino acids), order Cat.# X808X (see Products, Available Separately).
Features - Benefits
Save Time: You can generate protein in only two hours, as compared to days when using cell-based (E. coli) systems.
Choose Your Format: Use plasmid or PCR-generated templates to generate protein.
Achieve High Yields: Generate 10- to 20-fold more protein (10–100μg/ml) when compared to other cell-free systems.
Generate Usable Protein: Generate soluble, full-length protein and avoid problems associated with E. coli systems.
Applications
Protein:protein interaction.
Protein:nucleic acid interactions.
Protein:ligand interaction.
Enzyme assays.
Protein expression.
For more information, see the Protocols & Applications Guide.
References
Falah, N. et al. (2012) J. Virol. 86, 691–704.
Stempin, C.C. et al. (2011) J. Biol. Chem. 286, 37147–57.
Liu, W. et al. (2011) Plant Cell. 23, 3853–65.
Shi, X. et al. (2011) J. Biol. Chem. 286, 33401–8.
Zhao, L. et al. (2010) J. Struct. Funct. Genomics 11, 201–9.
Zhao, K.Q., Hurst, R., Slater, M.R. and Bulleit, R.F. (2007) J. Struct. Funct. Genomics 8, 199–208.
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