SS PLUS DIRECT CDNA SYSTEM30 RXNS

• 产品编号：L101506      品牌：invitrogen       原厂货号：L101506
• 产品分类：DNA和RNA纯化 > 核酸标记 > DNA标记
• 应用分类：

描述：

The SuperScript® Plus Direct cDNA Labeling System is a microarray labeling kit that combines the superior performance of SuperScript® III Reverse Transcriptase (RT) with the simplicity of direct labeling. In place of an enzyme spike-in, the streamlined protocol incorporates balanced sets of novel, array-optimized, nucleotides conjugated to Alexa Fluor® 555 and 647 dyes. This results in more reproducible array data with higher correlation coefficients than using standalone reagents. The SuperScript® Plus Direct cDNA Labeling System provides:

• Optimized, all-inclusive direct labeling with balanced aha-Alexa Fluor® nucleotides, enabling higher numbers of array positives
• Improved correlation coefficients for more accurate data (Figure 1)
• Low elution purification columns included for ease of use

In the SuperScript® Plus cDNA Labeling method, anchored oligo(dT) anneals to the mRNA template (Figure 2). SuperScript® III RT extends from the priming site, incorporating dUTP labeled with Alexa Fluor® dye directly to produce labeled cDNA. Template RNA is degraded by base hydrolysis. Free nucleotides and other contaminants are removed using low-elution volume purification columns before hybridization to the microarray.

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