MS IL 6 ELISA KIT

产品编号：KMC0062 品牌：invitrogen 原厂货号：KMC0062
产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
应用分类：

包装：

192 TESTS BIOSOURCE (TM)

运保温度:

2-8°C

到货周期：

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其它组分：

Ms IL-6 Standard, recombinant Ms IL-6. Contains 0.1% sodium azide. Refer to vial label for quantity andreconstitution volume. 【包装：4 vials】

Standard Diluent Buffer. Contains 0.1% sodium azide; 25 mL per bottle.【包装：2 bottles】

Ms IL-6 High and Low Control, recombinant Ms IL-6 in tissue culture matrix, lyophilized. Contains 0.1% sodium azide. Refer to vial label for quantity and reconstitution volume. Once reconstituted, aliquot andstore at –20ºC or below. Avoid repeated freeze-thawcycles. 【包装：2 vials】

Antibody Coated Wells. 12 x 8 Well Strips.【包装：2 plates】

Ms IL-6 Biotin Conjugate (Biotin-labeled anti-IL-6).Contains 0.1% sodium azide; 11 mL per bottle. 【包装：2 bottles】

Streptavidin-HRP (100X). Contains 3.3 mM thymol; 0.125 mL per vial.【包装：2 vials】

Streptavidin HRP Diluent. Contains 3.3 mM thymol; 25 mL per bottle.【包装：1 bottle】

Wash Buffer Concentrate (25X). 100 mL per bottle.【包装：1 bottle】

Stabilized Chromogen, Tetramethylbenzidine (TMB).25 mL per bottle. 【包装：1 bottle】

Stop Solution. 25 mL per bottle.【包装：1 bottle】

Plate Covers, adhesive strips.【包装：6】

描述：

The Mouse IL-6 ELISA research use only kit is designed to detect and quantify the level of mouse IL-6 in serum, plasma, and supernatant using 96-well plates and a microplate reader.

Easy to run
Rapid protocol
Convenient precoated, removable 8-well strips

Easy-to-Run Sandwich ELISA
The total assay incubation time is only 3.5 hours. A monoclonal capture antibody specific for Mouse IL-6 has been coated onto the wells of the 96-well plate provided. Samples, including a standard of known Mouse IL-6 content, controls, and unknowns, are pipetted into these wells followed by an addition of a biotinylated polyclonal detector antibody. During the first incubation, Mouse IL-6 antigen binds to the immobilized capture antibody and to the solution phase biotinylated detector antibody on a second site. After washing, a streptavidin-horseradish peroxidase (HRP) is added. This binds to the biotinylated detection antibody to complete 查看更多the four‑member sandwich. After a second incubation and washing to remove all unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Mouse IL-6 present in the original specimen and the optical density can be read on a standard microplate reader.

Ready-to-Use Mouse IL-6ELISA Kit Contains These Reagents, Buffers, and Supplies:

Mouse IL-6 Antibody Coated 96-Well Plate
Mouse IL-6 Standard
Standard Diluent Buffer
Mouse IL-6 Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer Concentrate (25X)
Stabilized Chromogen, TMB
Stop Solution
Plate Covers
Detailed protocol with validation tests

Convenient Kit Format
The kit comes with a capture antibody precoated to the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips allowing you to run as few samples as you want.

Rigorous Validation
Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency. See protocol insert for more information on validation.

Part of the ELISA Kit Family
We have a wide menu of Life Technologies ELISA research use only kits. With excellent sensitivity, proven specificity, and consistent performance, these optimized ELISA kits offer you a choice when it comes to getting physiologically relevant and quantitative results.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

注意事项：

Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.
Store Kit at 2-8°C.