The Mouse Interleukin-2 (IL-2, IL2) ELISA research-use-only kit is to be used for the quantitative determination of IL-2 in samples (see sample types indicated) using 96-well plates and a microplate reader. The assay will recognize both natural and recombinant forms of this target.
Performance characteristics
• Sensitivity: <4 pg/mL • Standard curve range: 15.6–1,000 pg/mL • Sample type(s): serum, plasma, cell culture supernatant • Specificity: natural and recombinant mouse IL-2 • Cross-reactivity: see kit manual for cross-species and/or cross-target reactivity • Sample volume: 50 μL • Total assay time: 3 hours
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Principle of the method
The mouse IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
Interleukin-2 (IL-2), also known as T Cell Growth Factor, is a 15.5 kDa glycoprotein secreted primarily by activated T lymphocytes. The protein consists of 133 amino acids and is the product of several post-translational processing steps. Transcription and release of synthesized IL-2 is stimulated by antigen or mitogen activation of mature T lymphocytes. IL-2 binds to cell surface receptors and promotes the clonal expansion of antigen-specific effector T cells. IL-2 is a T cell differentiation factor, able to induce the production of other lymphokines, such as IL-4 and interferon-γ. IL-2 promotes the growth of B cells and induction of immunoglobulin secretion. IL-2 stimulates J chain synthesis which leads to assembly and secretion of IgM. Myeloid cell populations such as macrophage precursors and primary peripheral blood monocytes express IL-2 receptors. The binding of IL-2 will cause, in these cells, proliferation, differentiation and enhancement of cytolytic activity.*
* Please refer to ELISA protocol for specific reference citations.
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