The JAK2 (Total) ELISA kit is designed to detect and quantify the level of JAK2 protein. This assay is intended for the detection of JAK2 from human cell lysates using 96-well plates and a microplate reader.
• Easy to run • More sensitive than Westerns • Convenient precoated, removable 8-well strips
Easy-to-Run Sandwich ELISA A monoclonal capture antibody specific for JAK2 has been coated onto the wells of the 96-well plate provided. Samples, including a standard of known JAK2 (Total) content, controls, and unknowns, are pipetted into these wells and then a rabbit detection antibody specific for JAK2 (Total) is added to the wells. During the first incubation, the JAK2 antigen binds to the immobilized capture antibody and the JAK2 rabbit antibody serves as a detection antibody by binding to the immobilized JAK2 (Total) protein. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of JAK2 (Total) present in the original specimen and the optical density can be read on a standard microplate reader. The total assay incubation time is only 4 hours.
Detect Low Expressing Proteins and Use Less Sample ELISA kits designed to measure intracellular signaling targets are 2–10X more sensitive than Western blotting. The improved sensitivity enables you to detect low-expressing proteins that otherwise may not be distinguishable from background. In addition, the amount of sample needed to run the assay is less than what is needed for Western blots.
Ready-to-Use JAK2 Total ELISA Kit Contains These Reagents, Buffers, and Supplies:
• JAK2 Antibody-Coated 96-Well Plate • JAK2 Total Standard • Standard Diluent Buffer • JAK2 Total Detection Antibody • anti-rabbit IgG-HRP (100X) • HRP Diluent • Wash Buffer Concentrate (25X) • Stabilized Chromogen, TMB • Stop Solution • Plate Covers • Detailed protocol with validation tests
Convenient Kit Format The kit comes with a capture antibody precoated to the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips allowing you to run as few samples as you want. In addition, the standards provided in the kit can be used to obtain quantitative results from the standard curve and/or can be used as a positive control.
Rigorous Validation Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency. See protocol insert for more information on validation.
Part of the ELISA Kit Family We have a wide array of ELISA kits targeted to disease-related proteins. With excellent sensitivity, proven specificity and consistent performance, these optimized ELISA kits offer you a choice when it comes to getting physiologically relevant and quantitative results.
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