The FAK (Total) ELISA kit is designed to detect and quantify the level of FAK protein. This assay is intended for the detection of FAK from human, rat and mouse cell lysates using 96-well plates and a microplate reader.
• Easy to run • More sensitive than Westerns • Convenient precoated, removable 8-well strips
Easy-to-Run Sandwich ELISA A monoclonal capture antibody specific for FAK has been coated onto the wells of the 96-well plate provided. Samples, including a standard of known FAK (Total) content, controls, and unknowns, are pipetted into these wells and then a rabbit detection antibody specific for FAK (Total) is added to the wells. During the first incubation, the FAKantigen binds to the immobilized capture antibody and the FAKrabbit antibody serves as a detection antibody by binding to the immobilized FAK (Total) protein. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of FAK (Total) present in the original specimen and the optical density can be read on a standard microplate reader. The total assay incubation time is only 4 hours.
Detect Low Expressing Proteins and Use Less Sample ELISA kits designed to measure intracellular signaling targets are 2–10X more sensitive than Western blotting. The improved sensitivity enables you to detect low-expressing proteins that otherwise may not be distinguishable from background. In addition, the amount of sample needed to run the assay is less than what is needed for Western blots.
Ready-to-Use FAK Total ELISA Kit Contains These Reagents, Buffers, and Supplies:
• FAK Antibody-Coated 96-Well Plate • FAK Total Standard • Standard Diluent Buffer • FAK Total Detection Antibody • anti-rabbit IgG-HRP (100X) • HRP Diluent • Wash Buffer Concentrate (25X) • Stabilized Chromogen, TMB • Stop Solution • Plate Covers • Detailed protocol with validation tests
Convenient Kit Format The kit comes with a capture antibody precoated to the plate, eliminating an overnight coating process. The plates are machine coated to provide low variability well-to-well. Furthermore, the plate comes as removable 8-well strips allowing you to run as few samples as you want. In addition, the standards provided in the kit can be used to obtain quantitative results from the standard curve and/or can be used as a positive control.
Rigorous Validation Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency. See protocol insert for more information on validation.
Part of the ELISA Kit Family We have a wide array of ELISA kits targeted to disease-related proteins. With excellent sensitivity, proven specificity and consistent performance, these optimized ELISA kits offer you a choice when it comes to getting physiologically relevant and quantitative results.
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