The Human Tumor Necrosis Factor alpha (TNF alpha, TNF) Ultrasensitive (US) ELISA research-use-only kit is to be used for the quantitative determination of TNF alpha in samples (see sample types indicated) using 96-well plates and a microplate reader. The assay will recognize both natural and recombinant forms of this target.
Performance characteristics
• Sensitivity: <0.09 pg/mL • Standard curve range: 0.5‒32 pg/mL • Sample type(s): serum, plasma, cell culture supernatant • Specificity: natural and recombinant human TNF alpha • Cross-reactivity: see kit manual for cross-species and/or cross-target reactivity • Sample volume: 50 μL • Total assay time: 3 hours
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Principle of the method
The human TNF alpha ultrasensitive solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Target information
Human Tumor Necrosis Factor-Alpha (TNF-α), also called cachectin, is a 157 AA nonglycosylated polypeptide cytokine mainly produced by activated macrophages. Lipopolysaccharide (LPS) is a potent stimulus for TNF-α production in macrophages. TNF-α is an important mediator of the in vivo effects of LPS. The various biological activities of TNF-α may be classified as:
- Immunomodulating and proinflammatory activities: TNF-α regulates the production of antibodies by B cells and stimulates cytotoxic T cells.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
