The Human Osteocalcin (OST) ELISA research-use-only kit is to be used for the quantitative determination of intact human osteocalcin in serum and plasma samples using 96-well plates and a microplate reader.
Performance characteristics • Sensitivity: 0.08 ng/mL • Standard curve range: 1.56‒75 ng/mL • Sample types: serum, plasma • Species cross-reactivity: human • Sample volume: 25 μL • Total assay incubation time: 2.5 hrs
Principle of the method The Human Osteocalcin ELISA is a solid phase enzyme-amplified sensitivity immunoassay (EASIA) performed on a microtiter plate. The assay uses monoclonal antibodies (MAbs) directed against distinct epitopes of human osteocalcin. Standards and samples react with the capture monoclonal antibody (MAb 1) coated on the microtiter well and with a monoclonal antibody (MAb 2) labeled with horseradish peroxidase (HRP). After an incubation period allowing the formation of a sandwich (coated MAb 1 - human osteocalcin - MAb 2 – HRP), the microtiter plate is washed to remove unbound enzyme-labeled antibody. Bound enzyme-labeled antibody is measured through a chromogenic reaction. Chromogenic solution (TMB, ready for use) is added and incubated. The reaction is stopped with the addition of Stop Solution and the microtiter plate is then read at the appropriate wavelength. The amount of substrate turnover is determined colorimetrically by measuring the absorbance which is proportional to the human osteocalcin concentration. A standard curve is plotted and human osteocalcin concentration in a sample is determined by interpolation from the standard curve.
Background information Osteocalcin or bone Gla protein (B.G.P.) is the major non-collagenous protein of the bone matrix. It has a molecular weight of 5,800 Da and contains 49 amino acids, including 3 residues of gamma carboxy-glutamic acid. Osteocalcin is synthesized in the bone by the osteoblasts. After production, it is partly incorporated in the bone matrix and the rest is found in the blood circulation. The exact physiological function of osteocalcin is still unclear. Studies show that the circulating levels of osteocalcin reflect the rate of bone formation.
Each ELISA kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency. See product insert for more information on validation.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
