The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of your gene of interest. This vector has the following features:
• The OpIE2 promoter for constitutive expression • The Zeocin™ resistance gene for rapid selection of stably transfected cell lines • C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin
In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistance protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines with Zeocin™ and confers a fluorescent phenotype that simplifies identification of transfected cells.
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For Research Use Only. Not for use in diagnostic procedures.