The Champion™ pET302/NT-His and pET303/CT-His Vector Kit is designed for cloning a gene of interest via restriction enzyme(s) and ligase (REaL) and subsequent high-level expression from the strong bacteriophage T7 promoter. In addition to the T7 promoter, each vector contains only the necessary functional elements and an N- or C-terminal 6xHis tag (pET302/NT-His and pET303/CT-His, respectively) for convenient purification and detection (Figure 1). Expression levels obtained from these vectors may be higher than those obtained from another suppliers pET vector (Figure 2). To maximize expression, use with MagicMedia™ E. coliExpression Medium.
Contents and Storage:
The Champion™ pET302/NT-His and pET303/CT-His Vector Kit includes 10 µg each of pET302/NT-His, pET303/CT-His, and control vectors. Store at -20“C. All components are guaranteed stable for 6 months when properly stored.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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