PENTR5-TOPO 20

产品编号：K59120 品牌：invitrogen 原厂货号：K59120
产品分类：克隆 > 克隆载体和克隆试剂盒 > 不依赖DNA连接酶的克隆试剂盒 > 其它克隆载体 > Gateway入门载体-pENTR
应用分类：暂定分类 > Gateway® 克隆 > 入门载体与试剂盒

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其它组分：

pENTR.5亴-TOPOR vector, TOPO®-adapted【包装：20 μl，，运保温度：-20°C】

10X PCR Buffer【包装：100 μl，，运保温度：-20°C】

dNTP Mix【包装：10 μl，，运保温度：-20°C】

Salt Solution【包装：50 μl，，运保温度：-20°C】

Sterile Water【包装：1 ml，，运保温度：-20°C】

GW1 Primer【包装：20 μl，，运保温度：-20°C】

GW3 Primer【包装：20 μl，，运保温度：-20°C】

Control PCR Primers【包装：10 μl，，运保温度：-20°C】

Control PCR Template【包装：10 μl，，运保温度：-20°C】

S.O.C. Medium【包装：6 ml，，运保温度：-80°C】

TOP10 cells【包装：21 x 50 μl，，运保温度：-80°C】

pUC19 Control DNA【包装：50 μl，，运保温度：-80°C】

描述：

The pENTR™ 5’-TOPO® TA Cloning® Kit combines Invitrogen’s TOPO® Cloning and MultiSite Gateway® Technologies to facilitate five-minute, one-step cloning of a Taq polymerase-amplified PCR product encoding a eukaryotic promoter of interest into a MultiSite Gateway® entry vector with ≥ 85% efficiency. Once cloned, your eukaryotic promoter may be transferred from the pENTR™ 5′-TOPO® vector to a suitable MultiSite Gateway® destination vector in a MultiSite Gateway® LR recombination reaction with other suitable entry clone(s) to generate an expression construct.

This kit can be used in combination with ViraPower™ HiPerform Promoterless Lentiviral Gateway® Vector Kit, or MultiSite Gateway® Three-Fragment Vector Construction Kit.

Advantages
• Choose the best promoter for your experiments and cell line by efficiently expressing your gene of interest from multiple promoter constructs
• When using in conjunction with ViraPower™ HiPerform™ Promoterless Lentiviral vector, you can 查看更多efficiently transduce and express your gene of interest driven by the promoter of choice in both dividing and non-dividing mammalian cells

Key Features
• Rapid PCR cloning with TOPO® cloning technology for cloning the 5’ element
• attR1-attR4 sites for compatibility with MultiSite Gateway® LR reaction for increased flexibility

Kit Includes
• pENTR™ 5´-TOPO® TA Cloning® Kit
• One Shot® TOP10 Chemically Competent E. coli (C4040-03)

Related SKUs
• ViraPower™ HiPerform™ Promoterless Lentiviral Gateway® Expression System (A111451)
• ViraPower™ HiPerform™ Promoterless Gateway® Vector Kit (A11146)
• MultiSite Gateway® Three-Fragment Vector Construction Kit (12537-023)

For research use only. Not intended for any therapeutic or diagnostic use.

注意事项：

The pENTR™ 5´-TOPO® TA Cloning® Kit with One Shot® TOP10 Chemically Competent E. coli contains two boxes.

The TOPO® Cloning box contains enough linearized and topoisomerase I-activated pENTR™ 5´-TOPO® vector for 20 cloning reactions, dNTPs, salt solution, control template and primers and primers for sequencing. Store at -20°C.

The One Shot® TOP10 box contains transformation reagents including 20 single-use 50 µl aliquots of One Shot® Competent Cells, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C.