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PLENTI6.3/V5-TOPO TA CLONING COMBO KIT

 
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描述:

Invitrogen's™ pLenti6.3⁄V5™-TOPO®Cloning Kit is part of our ViraPower™ HiPerform™ Lentiviral Expression System (catalog K5310-00). 

The pLenti6.3⁄V5™-TOPO® -TA Cloning Kit contains the TOPO®- adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5™-TOPO® for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5™-TOPO® vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages
• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• 5 minute, high-efficiency TOPO® PCR cloning in three simple steps

Key Features
• HiPerform™ WPRE and cPPT elements
• CMV promoter 
• V5 epitope tag at C terminus
• Blasticidin selection

Kit includes
• A lacZ vector as a positive control, pLenti6.3⁄V5-GW⁄lacZ™
• Stbl3™ competent cells

For research use only. Not intended for any therapeutic or diagnostic use.


注意事项:

For Research Use Only. Not for use in diagnostic procedures.


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