NT-GFP FUSION TOPO CLON KIT 20 RXN

产品编号：K481001 品牌：invitrogen 原厂货号：K481001
产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 哺乳动物表达载体
应用分类：

包装：

运保温度:

到货周期：

登录后查看

标准价：

￥客户可见

会员价：

￥客户可见

积分：

客户可见分

登录之后可加入购物车购买，请您

运费与支付说明：

1.含干冰类产品有运费；

2. 必须现金付款或有信用额度的会员才可以直接发货，否则需要等待现金付款信息。

其它组分：

pcDNA3.1/NT-GFP-TOPO【包装：20 μl，，运保温度：-20°C】

10X PCR Buffer【包装：100 μl，，运保温度：-20°C】

dNTP Mix【包装：10 μl，，运保温度：-20°C】

Salt Solution【包装：50 μl，，运保温度：-20°C】

Sterile Water【包装：1 ml，，运保温度：-20°C】

Control PCR Template【包装：10 μl，，运保温度：-20°C】

Control PCR Primers【包装：10 μl，，运保温度：-20°C】

Expression Control Plasmid【包装：10 μl，，运保温度：-20°C】

Forward Sequencing Primer【包装：20 μl，，运保温度：-20°C】

Reverse Sequencing Primer【包装：20 μl，，运保温度：-20°C】

SOC Medium【包装：6 ml，，运保温度：-80°C】

TOP10 cells【包装：21 x 50 μl，，运保温度：-80°C】

pUC19 Control DNA【包装：50 μl，，运保温度：-80°C】

描述：

The GFP Fusion TOPO® TA Expression Kits are designed to allow fusion of a protein of interest to the Cycle 3 GFP protein. These kits provide topoisomerase-I activated pcDNA3.1/NT-GFP-TOPO® or pcDNA3.1/CT-GFP-TOPO® vectors (Figure 1). Taq-amplified DNA fragments are ligated into these vectors in a simple 5-minute reaction right on your bench top. The vectors are designed for high-level expression of transient or stable GFP fusion proteins in a wide range of mammalian cells (Figure 2). Expression is easily detected in living cells using fluorescence. In addition, recombinant proteins expressed from these vectors can be detected on western blots using GFP Antiserum.

注意事项：

GFP Fusion TOPO® Expression Kits contain two boxes. The GFP Fusion TOPO® TA Cloning box contains linearized and topoisomerase I-activated cloning vector, dNTPs, salt solution control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. The One Shot ¤ box contains transformation reagents including single-use, 50-µl aliquots of One Shot® TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid. Store the One Shot® box at -80°C. Kits are guaranteed stable for 6 months when properly stored.