SC-PCDNA3.1/V5-HIS-TOPO TA20 RXN

产品编号：K480001SC 品牌：invitrogen 原厂货号：K480001SC
产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 哺乳动物表达载体
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包装：

20 RXN

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描述：

The pcDNA™3.1⁄V5-His TOPO® TA Expression Kit offers one-step cloning of Taq-amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3.1⁄V5-His-TOPO® vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants. This kit is specifically formatted for storage in Invitrogen Supply Centers at -80°C. In addition, the vector includes the following features: Strong CMV promoter for high-level, constitutive expression V5 epitope tag for efficient detection of recombinant proteins 6×His sequence for purification using nickel-chelating resin and detection

注意事项：

Each pcDNA™3.1 Directional TOPO¤ Expression Kit contains two boxes. The TOPO® box contains linearized and topoisomerase I-activated pcDNA3.1D/V5-His-TOPO® vector, dNTPs, control template and primers, primers for sequencing, and an expression control plasmid. Store at -20°C. The One Shot® box contains single-use 50-µl aliquots of One Shot® TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C. Guaranteed stable for 6 months when properly stored.