TOPO® TA Cloning® Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes (1). They use a pCR®-TOPO® Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR®-TOPO® Vectors include: • 3´-T overhangs for direct ligation of Taq-amplified PCR products • Choice of T7 (pCR®2.1-TOPO®) or T7 and SP6 (pCR®II-TOPO®) promoters for in vitroRNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing. • EcoR I sites flanking the PCR product insertion site for easy excision of inserts • Kanamycin and ampicillin resistance genes for your choice of selection in E. coli • Easy blue/white colony screening for selection of recombinants TOPO® TA Cloning® Kits also available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO®-cloned inserts for downstream analysis.
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For Research Use Only. Not for use in diagnostic procedures.