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PBAD TOPO TA CLONING KIT 40 RXN

PBAD TOPO TA CLONING KIT 40 RXN

产品编号：K430040 品牌：invitrogen 原厂货号：K430040
产品分类：蛋白质表达与纯化 > 蛋白质表达系统 > 细菌表达系统
应用分类：

包装：

运保温度:

room temperature

到货周期：

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其它组分：

pBAD-TOPO?vector【包装：25 μL，，运保温度：–20°C】

10X PCR Buffer【包装：100 μL，，运保温度：–20°C】

dNTP Mix【包装：10 μL，，运保温度：–20°C】

20% L-Arabinose【包装：1 mL，，运保温度：–20°C】

pBAD Forward Sequencing Primer【包装：20 μL，，运保温度：–20°C】

pBAD Reverse Sequencing Primer【包装：20 μL，，运保温度：–20°C】

Salt Solution【包装：50 μL，，运保温度：–20°C】

Control PCR Primers【包装：10 μL，，运保温度：–20°C】

Control PCR Template【包装：10 μL，，运保温度：–20°C】

Sterile Water【包装：1 mL，，运保温度：–20°C】

Expression Control Plasmid【包装：10 μL，，运保温度：–20°C】

TOP10 Cells【包装：21 別 50 L，，运保温度：–80°C】

S.O.C. Medium【包装：6 mL，，运保温度：–80°C】

pUC19 Control DNA【包装：50 μL，，运保温度：–80°C】

LMG194 E. coli stab【包装：a small bag，，运保温度：4°C】

描述：

The pBAD TOPO® TA Expression Kit is specifically designed for one-step cloning and regulated prokaryotic expression of Taq-amplified PCR products. Once you've TOPO® Cloned your PCR product, you can go straight to protein expression. Some of the convenient features of the pBAD-TOPO® vector include:

Linearized, topoisomerase I-activated vector for 5-minute cloning of Taqamplified PCR products

The araBAD promoter for tightly regulated expression in E. coli

V5 epitope tag for detection with an Anti-V5 Antibody

C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with an Anti-His(C-term) Antibody

Enterokinase cleavage site for removal of N-terminal leader peptide

注意事项：

Each pBAD TOPO® TA Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD TOPO® TA box contains all of the reagents necessary for PCR (except Taq polymerase), including 200 ng of topoisomerase I-activated pBAD-TOPO® vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot® box contains all of the reagents necessary for transformation, including single-use 50 µl aliquots of One Shot® TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot® cells at -80°C. Store the LMG194 E. coli stab at room temperature. All reagents are guaranteed stable for 6 months when properly stored.