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CHAMPION PET SUMO EXPRESSION # 20 REACTIONS

CHAMPION PET SUMO EXPRESSION # 20 REACTIONS

产品编号：K30001 品牌：invitrogen 原厂货号：K30001
产品分类：蛋白质表达与纯化 > 蛋白质表达系统 > 细菌表达系统
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其它组分：

pET SUMO vector,linearized 【包装：5 x 10 μl，，运保温度：-20°C】

10X PCR Buffer【包装：100 μl，，运保温度：-20°C】

dNTP Mix【包装：10 μl，，运保温度：-20°C】

10X Ligation Buffer【包装：100 μl，，运保温度：-20°C】

T4 DNA Ligase【包装：25 μl，，运保温度：-20°C】

Sterile Water【包装：1 ml，，运保温度：-20°C】

SUMO Forward SequencingPrimer 【包装：10 μl，，运保温度：-20°C】

T7 Reverse Sequencing Primer【包装：20 μl，，运保温度：-20°C】

Control PCR Primers【包装：10 μl，，运保温度：-20°C】

Control PCR Template【包装：10 μl，，运保温度：-20°C】

pET SUMO/CAT【包装：10 μl，，运保温度：-20°C】

S.O.C. Medium【包装：6 ml，，运保温度：-80°C】

Mach1™-T1R cells【包装：21 x 50 μl，，运保温度：-80°C】

pUC19 Control DNA【包装：50 μl，，运保温度：-80°C】

S.O.C. Medium【包装：6 ml，，运保温度：-80°C】

BL21(DE3) Cells【包装：21 x 50 μl，，运保温度：-80°C】

pUC19 Control DNA【包装：50 μl，，运保温度：-80°C】

SUMO Protease (1 U/μl)【包装：5 x 50 μl，，运保温度：-20ºC】

10X SUMO Protease Buffer + Salt【包装：500 μl，，运保温度：-20ºC】

10X SUMO Protease Buffer – Salt【包装：500 μl，，运保温度：-20ºC】

描述：

The Champion™ pET SUMO Expression System produces the highest levels of soluble protein in E. coli. It utilizes a small ubiquitin-related modifier (SUMO) fusion, belonging to the growing family of ubiquitin-related proteins, to enhance the solubility of expressed fusion proteins. In contrast to ubiquitin, SUMO is involved in the stabilization and localization of proteins in vivo. After expression, the 11 kd SUMO moiety can be cleaved by the highly specific and active SUMO (ULP-1) protease at the carboxyl terminal, producing a native protein*. The Champion™ pET SUMO Protein and Peptide Expression System offers:

Greatly enhanced solubility with an N-terminal SUMO fusionHighly efficient cleavage- produces native protein of interest with SUMO (ULP-1) protease*Highly specific cleavage- eliminates the chance of your protein of interest being internally digested, regardless of its amino acid sequenceSignificantly increased stability with SUMO fusion-can be used for small

peptide productionT7lac promoter for high-level protein expressionN-terminal 6xHis tag for protein detection and purification

注意事项：

The Champion™ pET SUMO Expression System is provided as a complete system. The Champion™ pET SUMO TA Cloning® box contains linearized Champion™ pET SUMO vector, sterile water, dNTPs, 10X PCR Buffer, control template and primers, T4 DNA ligase, 10X ligation buffer, primers for sequencing or PCR screening, and an expression control. Store at -20°C. The SUMO Protease box contains SUMO Protease and buffers. Store at -80°C. The One Shot® TOP10 box contains twenty-one 50 µl aliquots of chemically competent E. coli, S.O.C. medium, and a control plasmid. Store at -80°C. The One Shot¤ BL21(DE3) box contains twenty-one 50 µl aliquots of chemically competent E. coli, S.O.C. medium, and a control plasmid. Store at -80°C.