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PENTR/TEV/D-TOPO CLONING KIT 20 REACTIONS

 
包装:
运保温度:
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1.含干冰类产品有运费;

2. 必须现金付款或有信用额度的会员才可以直接发货,否则需要等待现金付款信息。

其它组分:

pENTR™ TOPO® vector,TOPO®-adapted【包装:20 μl,,运保温度:-20°C】
dNTP Mix【包装:10 μl,,运保温度:-20°C】
Salt Solution【包装:50 μl,,运保温度:-20°C】
Water【包装:1 ml,,运保温度:-20°C】
M13 Forward (-20) Sequencing Primer【包装:20 μl,,运保温度:-20°C】
M13 Reverse Sequencing Primer【包装:20 μl,,运保温度:-20°C】
Control PCR Primers【包装:10 μl,,运保温度:-20°C】
Control PCR Template【包装:10 μl,,运保温度:-20°C】
S.O.C. Medium【包装:6 ml,,运保温度:-80°C】
TOP10 or Mach1™-T1R cells【包装:21 x 50 μl,,运保温度:-80°C】
pUC19 Control DNA【包装:50 μl,,运保温度:-80°C】


描述:

The pENTR™⁄TEV⁄D-TOPO® Cloning Kits utilize a highly efficient, 5-minute cloning strategy ("TOPO® Cloning") to directionally clone a blunt-end PCR product into a vector for entry into the Gateway® System or the MultiSite Gateway® System. Blunt-end PCR products clone directionally into the pENTR™⁄TEV⁄D-TOPO® entry vector at greater than 90% efficiency, and include a TEV protease-dependent cleavage site for removal of N-terminal tags from expressed proteins. The kit comes with everything necessary to clone and select your PCR amplified gene of interest.


注意事项:

pENTR™⁄TEV⁄D-TOPO® Cloning Kit contains pENTR⁄TEV⁄D-TOPO® vector, dNTPs, salt solution, sterile water, universal M13 sequencing primers, OneShot® TOP10 Chemically E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store Competent E. coli at -80°C. Store all other components at -20°C.


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