TOPO® TA Cloning® Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes. TOPO® TA Cloning® Kits use a pCR™-TOPO® Vector with covalently bound topoisomerase I for fast cloning and recombinants. The newest TOPO® TA Cloning® Kit comes with a Gateway® (GW) entry vector, pCR™8/GW/TOPO® (Figure 1). This vector has:
• 3´-T overhangs for direct ligation of Taq-amplified PCR products • att sites for rapid recombination into a variety of Gateway® destination vectors • Novel GW primer sites, within the att regions, located less than 55 base pairs from the PCR product insertion site for convenient sequencing • Spectinomycin resistance gene for robust selection in E. coli • EcoR I sites flanking the PCR product insertion site for easy excision of inserts
Selected TOPO® TA Cloning® Kits for entry into the Gateway® Technology are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO®-cloned inserts for downstream analysis.
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注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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