The pCR™8/GW/TOPO® TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway® recombination cloning vector platform. Our pCR™8 TOPO® TA vector contains a minimal MCS, so you sequence more of your insert and less of the vector. And with our patented att sites, the pCR™8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway® recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time.
The pCR™8/GW/TOPO® TA Cloning Kit features: • Fast growing E. coli that shortens cloning time • Maximum convenience: includes plasmid miniprep kit • att sites for rapid recombination into any Gateway® destination vector • 3'-T overhangs for direct ligation of Taq-amplified PCR products • Sequencing primer sites within 55 bp of insertion site ensures you sequence more insert, less vector • Spectinomycin resistance gene for robust selection in E. coli • EcoR I sites flanking the PCR product insertion site for easy excision of inserts
This kit includes One Shot® Mach1™-T1R Chemically Competent E. coli and a PureLink® Quick Plasmid Miniprep Kit.
原厂资料：
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
