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PCR8/GW/TOPO MINIPREP MACH1 20 PREPS

 
包装:
运保温度: -20°C
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描述:

The pCR™8/GW/TOPO® TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway® recombination cloning vector platform. Our pCR™8 TOPO® TA vector contains a minimal MCS, so you sequence more of your insert and less of the vector. And with our patented att sites, the pCR™8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway® recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time.

The pCR™8/GW/TOPO® TA Cloning Kit features:
• Fast growing E. coli that shortens cloning time
• Maximum convenience: includes plasmid miniprep kit
• att sites for rapid recombination into any Gateway® destination vector
• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• Sequencing primer sites within 55 bp of insertion site ensures you sequence more insert, less vector
• Spectinomycin resistance gene for robust selection in E. coli
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts

This kit includes One Shot® Mach1™-T1R Chemically Competent E. coli and a PureLink® Quick Plasmid Miniprep Kit.


注意事项:

For Research Use Only. Not for use in diagnostic procedures.
 


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