PCR2.1 VECTOR 40 REACTIONS

产品编号：K202040 品牌：invitrogen 原厂货号：K202040
产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > PCR克隆载体
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运保温度:

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其它组分：

pCR®2.1, linearized【包装：5 x 10 μl，，运保温度：-20ºC】

10X PCR Buffer【包装：100 μl，，运保温度：-20ºC】

10X Ligation Buffer【包装：100 μl，，运保温度：-20ºC】

50 mM dNTPs【包装：10 μl，，运保温度：-20ºC】

T4 DNA Ligase【包装：25 μl，，运保温度：-20ºC】

Sterile Water【包装：1 ml，，运保温度：-20ºC】

Control DNA Template【包装：10 μl，，运保温度：-20ºC】

Control PCR Primers【包装：10 μl，，运保温度：-20ºC】

描述：

TA Cloning® Kits are designed for cloning Taq-amplified PCR products directly from a PCR reaction using an overnight ligation step. The kits use a pCR® vector and yield >=80% recombinants with >=90% of the recombinants containing inserts. Some of the convenient features of the pCR® vectors are:

3´-T overhangs for direct ligation of Taq-amplified PCR products

Choice of T7 (pCR®2.1) or T7 and Sp6 (pCR®II) promoters for in vitro RNA transcription and sequencing

Versatile polylinker with flanking EcoR I sites for easy excision of inserts

Choice of kanamycin or ampicillin selection

Easy blue/white colony screening

M13 forward and reverse primer sites for sequencing

注意事项：

Forty reaction kits are supplied as two 20 reaction kits.

TA Cloning® Kits contain linearized pCR®2.1 or pCR®II vector, T4 DNA ligase, 10X ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Competent cell kits contain One Shot® Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot® E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.