TREX G2A NFAT BLA FS 293F CELL KIT

• 产品编号：K1749      品牌：invitrogen       原厂货号：K1749
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > GPCR检测试剂盒
• 应用分类：行业及应用 > 药物研发 > 靶标和先导药物鉴定及验证 > G 蛋白偶联受体生物学G-Protein Coupled Receptor Biology (GPCR) > Cell-Based GPCR Reporter Assays > 试剂盒和分析 > 基于细胞的分析试剂盒 > 基于细胞的 GPCR 分析试剂盒
  　　　　　行业及应用 > 药物研发 > 靶标和先导药物鉴定及验证 > G 蛋白偶联受体生物学G-Protein Coupled Receptor Biology (GPCR) > Cell-Based GPCR Reporter Assays > Cell-Based GPCR Reporter Assays - G-Protein Coupled Receptors > VIP & PACAP > GPR132 (G2A)

描述：

GeneBLAzer® T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells contain the human GPR132 (G2A), (Accession # NP_037477) stably integrated into the CellSensor® NFAT-bla Freestyle HEK 293F cell line. CellSensor® NFAT-bla Freestyle HEK 293F cells (Cat. no. K1725) contain a beta-lactamase reporter gene under control of the NFAT Response Element.

The GeneBLAzer® T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells are functionally validated for Z' and EC50 concentrations of dDAVP. In addition, GeneBLAzer® GeneBLAzer® T-REx™ G2A-NFAT-bla Freestyle HEK 293F cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

G2A is an orphan GPCR. Upon overexpession G2A is constitutively active. This is an assay for the orphan GPCR based upon constitutive activity. To use the assay to detect inverse agonists, high amounts of doxycycline should be added. To use the assay to detect agonists, low amounts of doxycycline should be added. 

原厂资料：

注意事项：

For Research Use Only. Not for use in diagnostic procedures.