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注意事项:
The EasySelect Pichia Expression Kit includes 20 µg each of the plasmids pPICZ A, B, & C and pPICZα A, B, & C; Pichia pastoris strains X-33 (wild-type), GS115 (his4), KM71H (arg4 aox1::ARG4), and control strains for both intracellular and secreted expression; 250 mg Zeocin™ antibiotic; media (YP and YNB), EasyComp™ Pichia Transformation Kit, 2 µg each 5´ AOX1, 3´ AOX1, and α-factor sequencing primers. The pPICZ and pPICZα vectors are also available separately. A lacZ positive expression control is provided with pPICZ.
Store strains at +4°C. Store vectors and primers at -20°C. All components are guaranteed stable for 6 months when properly stored.
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