B-GAL ASSAY KIT

10X PBS (Phosphate Buffered Saline)【包装：60 ml ，，运保温度：RT or 4°C】

1X Lysis Buffer【包装：10 ml ，，运保温度：RT or 4°C】

10X Cleavage Buffer【包装：25 ml ，，运保温度：4°C】

β-mercaptoethanol【包装：750 μl，，运保温度：RT or 4°C】

ONPG【包装：5 x 2 ml，，运保温度：-20°C 】

Stop Buffer【包装：55 ml，，运保温度：RT or 4°C】

The β-Gal Assay Kit provides the reagents required to quickly measure the levels of active β-galactosidase expressed in cells transfected with plasmids expressing the lacZ gene. lacZ is a bacterial gene often used as a reporter construct in eukaryotic transfection experiments. The gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates and its activity is easily assayed. β-galactosidase catalyzes the hydrolysis of β-galactosidase such as ortho-nitrophenyl-D-galactopyranoside (ONPG). Hydrolysis of ONPG to the ONP anion produces a bright yellow color with a peak absorbance at 420 nm that can be quantified using a spectrophotometer.
10.7 gene expression