收藏本站
热门搜索: A3500  E1910  A1360  25200056  dmem  

H2 HEK 293T DA ASSAY KIT 1 PLATE

 
包装:
运保温度: liquid nitrogen
到货周期: 登录后查看
标准价: ¥客户可见
会员价: ¥客户可见
积分: 客户可见
 
登录之后可加入购物车购买,请您 
对比 收藏

运费与支付说明:

1.含干冰类产品有运费;

2. 必须现金付款或有信用额度的会员才可以直接发货,否则需要等待现金付款信息。

描述:

This cell line is available as dividing cells by requesting a quote for K1226 from dividingcells@invitrogen.com

GeneBLAzer® H2 HEK 293T DA (Division Arrested) cells and H2-CRE-blaHEK 293T cells contain the human Histamine Subtype 2 receptor (H2) stably integrated into the CellSensor® CRE-bla HEK 293T cell line. CellSensor® CRE-bla HEK 293T cells (Cat. no.K1112) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE). Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both H2 HEK 293T DA cells and H2-CRE-bla HEK 293T cells are functionally validated for Z’-factor and EC50 concentrations of Histamine (Figure 1). In addition, H2-CRE-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Histamine 2 (H2) receptors have been implicated in a number of disorders, including peptic ulcer disease (PUD), gastroesophageal reflux disease (GERD), dyspepsia, and stress ulcer prophylaxis.


Storage:

GeneBLAzer® H2 HEK 293T DA Cells and H2-CRE-bla HEK 293T Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.


Contents:
GeneBLAzer® H2 HEK 293T DA Assay Kit

Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).Includes:
•H2 HEK 293T DA cells 
•LiveBLAzer™-FRET B/G Loading Kit, 70µg
•Solution D, 1ml
•2 protocols
•Certificate of Analysis 
GeneBLAzer® H2 HEK 293T DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). 
Includes:
•H2 HEK 293T DA cells 
•1 protocol
•Certificate of Analysis
GeneBLAzer® H2-CRE-bla HEK 293T cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). 
Includes:
•H2-CRE-bla HEK 293T cells 
•1 protocol
•Certificate of Analysis


注意事项:

For Research Use Only. Not for use in diagnostic procedures.


相关图片

本产品可用于的实验

购此产品的人还购买了
最近浏览产品

京ICP备15036693号-2    京公网安备11010802025653    版权所有:北京逸优科技有限公司       0.04

 纠 错