Figure1. miRNA inhibitor function for miR-22 was assayed in HeLa cells 48 h after transfection of mature antisense or miRIDIAN microRNA Inhibitor (0.06 - 50 nM) using a dual luciferase reporter system. Control samples received no inhibitors.
Figure 2. miRIDIAN microRNA Inhibitor function for 7 human miRNAs was assayed in HeLa, HEK293, and MCF7 cells 48 h after transfection of 50 nM inhibitor using a dual luciferase reporter system. Control samples received no inhibitors.
产品用途：
通过miRIDIAN microRNA阻遏物抑制miRNA活性从而研究基因沉默效应
检测miRNAs所调节的基因表达和对信号通路的影响效应
阐明miRNA在诸如细胞发育、增殖、分化和凋亡中的机理
鉴定和确认miRNA靶标
原厂资料：
Thermo Scientific miRIDIAN microRNA Hairpin Inhibitors are the newest generation proprietary, synthetic inhibitors targeting all human, mouse, and rat microRNAs in the miRBase sequence database 19.0. miRIDIAN Hairpin Inhibitors are single-stranded, chemically-enhanced RNA oligonucleotides designed to bind and to sequester the complimentary, mature microRNA strand. Our proprietary inhibitor design allows for microRNA inhibition to be maintained longer than with any other synthetic inhibitor available on the market today.
Highlights
• Most effective inhibition of endogenous mature microRNA function by means of proprietary design (see Figure 1, Supporting Data)
• Patent-pending molecule combines chemical modifications and completely novel secondary structure motif
• Enhanced potency and longevity allows for multiplexed microRNA inhibition at very low nanomolar concentrations and with minimal toxicity (see Figure 2, Supporting Data)
Applications
• Suppress microRNA activity to study loss-of-function effects
• Screen for microRNAs that regulate gene expression and affect cellular processes
• Elucidate microRNA involvement in normal biological and disease pathways
• Identify and validate microRNA targets
• Inhibit microRNA clusters for combinatorial analysis (see Figure 3, Supporting Data)
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