MAGIC RED? CASPASE-3/7 KIT

The Magic Red^™ Caspase 3 & 7 kit uses a quick and easy method to analyze intracellular active caspases in apoptotic cells.

Magic Red^™ MR-(DEVD)₂ Reagent - lyophilized 
Hoechst 33342 Stain, 1 mL 
Acridine orange (AO), 0.5 mL

Caspase Magic Red^™ kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of caspases to produce a fluorescent product. The Magic Red^™ reagent contains a caspase inhibitor sequence (DEVD)₂ linked to a red (Cresyl Violet) fluorescent probe.

The Caspase Magic Red^™ kits are suitable for cells in suspension, adherent cells, thin frozen tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits. 

This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.

Store the unopened kit (and each unopened component) at +4^oC until the expiration date.
Protect the MR-(DEVD)₂ reagent from light at all times.

Once reconstituted, the 155X MR-(DEVD)₂ stock should be stored at -20°C protected from light.

The 155X MR-(DEVD)₂ reconstituted stock is stable for up to 6 months and may be thawed twice during that time.

Application

Application Name	Yes	No	Not Determined	Suggested Dilution
Immunofluorescence

Instructions For Use

 

MSDS

Health And Safety Information

Acknowledgements

Regulatory