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FAM FLICA? POLY CASPASE KIT

 
包装: 25 TESTS
运保温度: +4oC
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其它组分:

FAM-VAD-FMK FLICA™ Reagent - lyophilized 【包装:1 vial】
10X Wash Buffer【包装:15 mL 】
Fixative【包装:6 mL 】
Propidium Iodide【子货号: ,包装:1 mL】
Hoechst Stain【包装:1 mL 】


描述:

Product Information

The FAM FLICA Poly Caspase kit uses a quick and easy method to analyze active caspases in apoptotic cells.
This FLICA reagent binds the following activated caspases: Caspase 1, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8 and Caspase 9. It can also be used as a generic probe for the detection of most caspases.

Reagents In The Kit

1 vial of FAM-VAD-FMK FLICA Reagent - lyophilized 
10X Wash Buffer, 15 mL 
Fixative, 6 mL
Propidium Iodide, 1 mL 
Hoechst Stain, 1 mL 

Test Principle

Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.

The Caspase FLICA Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. 

This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.

Storage

Store the unopened kit (and each unopened component) at +4oC until the expiration date.
Protect the FLICA reagent from light at all times.

Once reconstituted, the 150X FLICA stock should be stored at -20°C protected from light.

Shelf Life

The 150X FLICA reconstituted stock is stable for up to 6 months and may be thawed twice during that time.

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name Yes No Not Determined Suggested Dilution
Flow Cytometry        
Immunofluorescence        
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
 

Instructions For Use

Instructions for use can be found at www.abdserotec.com/uploads/IFU/ICT091.pdf

 

MSDS

Health And Safety Information

Acknowledgements

FLICA is a trademark of Immunochemistry Technologies, LLC.

Licensed Use

Please see PDF datasheet for full Health & Safety information.

Regulatory

For research purposes only

 


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