The iBlot® 2 Transfer Stacks are used to transfer proteins using the iBlot® 2 Gel Transfer Device. The iBlot® 2 Transfer Stacks are consumable stacks with integrated PVDF or nitrocellulose transfer membranes for dry blotting of proteins. Each iBlot® 2 Transfer Stack contains a copper coated electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins.
Features of the iBlot® 2 Transfer Stacks:
• Increased shelf life • Reduced and compact packaging • Complete compatibility with iBlot® 2 Gel Transfer Device
PVDF Membrane The PVDF membrane (0.2 µm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. The PVDF membrane is preactivated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling. The proteins bind to the membrane due to hydrophobic interactions. The protein-binding capacity is 240 µg/cm2.
Transfer Stack Sizes The regular size (13 cm x 8.3 cm) of this stack is used for all Novex® midi gels (or other midi gel types), two Novex® mini gels (or other mini gel types), and E-PAGE™ 48 or 96 gels. The mini size (8 cm x 8 cm) (available separately) is used for all Novex® mini gels (or other mini gel types).
原厂资料:
The iBlot® 2 Transfer Stacks are used to transfer proteins using the iBlot® 2 Gel Transfer Device. The iBlot® 2 Transfer Stacks are consumable stacks with integrated PVDF or nitrocellulose transfer membranes for dry blotting of proteins. Each iBlot® 2 Transfer Stack contains a copper coated electrode and appropriate cathode and anode buffers in the gel matrix to allow fast, reliable transfer of proteins.
Features of the iBlot® 2 Transfer Stacks:
• Increased shelf life • Reduced and compact packaging • Complete compatibility with iBlot® 2 Gel Transfer Device
PVDF Membrane The PVDF membrane (0.2 µm pore size, low fluorescence) in this stack has higher binding capacity than nitrocellulose. The PVDF membrane is preactivated and ready for use without any pretreatment with alcohols. The membrane is compatible with commonly used detection methods such as staining, immunodetection, fluorescence, or radiolabeling. The proteins bind to the membrane due to hydrophobic interactions. The protein-binding capacity is 240 µg/cm2.
Transfer Stack Sizes The regular size (13 cm x 8.3 cm) of this stack is used for all Novex® midi gels (or other midi gel types), two Novex® mini gels (or other mini gel types), and E-PAGE™ 48 or 96 gels. The mini size (8 cm x 8 cm) (available separately) is used for all Novex® mini gels (or other mini gel types).