Image-iT® FX signal enhancer is a liquid that is applied directly to slides or coverslips containing fixed and permeabilized cell or tissue samples prior to staining with fluorescent probes. When Image-iT® FX signal enhancer is used, nonspecific fluorescence (background) commonly seen with the application of fluorescent conjugates of streptavidin, goat anti-mouse, or goat anti-rabbit IgG is largely eliminated.
Key Attributes
• Easy protocol—apply enhancer from ready-made solution in dropper bottle • Effective with many fluorescent labels [see Table 2 in the product manual] • Cost effective—supplies enough for 50 standard coverslips • Compatible with your immunostaining protocol’s other blocking steps
Reduce Nonspecific Binding of Dyes to Tissues. Alexa Fluor® dyes and many other dyes have negatively charged modifications that can lead to nonspecific association with cells and tissue. While not always significant, it can be a problem when pushing the limits of sensitivity in low-abundance targets. Image-iT® FX signal enhancer can significantly reduce this binding, resulting in marked improvements in staining.
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