HCA156 recognises LDH (L-Lactose Dehydrogenase) from the Malaria causing protozoan speciesPlasmodium vivax. It was shown to cross-react withPlasmodium falciparumLDH.
Clone 13980 was selected by phage display under heat stress conditions. The Tm value for thermal denaturation of the antibody was measured by CD spectroscopy and has been found to be 70 °C.
HCA156 is designed for use as a capture antibody with HCA155P as the detection antibody in sandwich assay development.
Product Form
Human IgG1 antibody selected from the HuCAL ® phage display library and expressed in a human cell line. This product is supplied as a liquid.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
This product is suitable for use in indirect ELISA applications. This product may be used as a capture reagent in a sandwich ELISA together withMCA155Pas the detection reagent.
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