HCA145A488 recognises the alpha chain of the human interleukin-7 receptor (IL-7R), which is also known as CD127.
CD127 is a 65-75kD transmembrane glycoprotein that associates with the interleukin-2 receptor gamma chain to form the functional high affinity IL-7 receptor. CD127 is expressed on lymphoid precursors in the bone marrow, pro-B cells, a subpopulation of thymocytes, T-cells and monocytes. CD127 is the specific receptor for IL-7 and the IL-7 receptor plays a critical role in lymphoid development.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a DYKDDDDK tag and a Strep tag at the C-terminus of the antibody heavy chain. This antibody is supplied conjugated to Alexa Fluor 488 - liquid
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit
www.abdserotec.com/protocols.
|
Application Name |
Yes |
No |
Not Determined |
Suggested Dilution |
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Flow Cytometry |
|
|
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Neat - |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
UniProt
Health And Safety Information
Acknowledgements
Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by AbD Serotec.
Regulatory
For research purposes only
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