HCA128A recognises theSchistosoma mansoniTetraspanning Orphan Receptor (Sm-4TOR), a 55kDa protein found on the tegumental surface ofS.mansonicercariae.
S.mansoniis one of 5 species of parasitic worm responsible for causing the water borne disease, schistosomiasis (also known as bilharzia) in humans.S.mansonihas a complex life cycle involving a primary (human) and intermediate (water snail) host.
Research has demonstrated that theS.mansoniTOR consists of 4 exons and is expressed at various developmental stages ofS.mansoni, however highest expression is at the cercariae (larval stage). It has therefore been hypothosized that as it is the larval stage (cercariae) ofS.mansoniwhich enters its human host through the skin, that Sm-4TOR expression may confer protection against complement attack from the hosts immune defence.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the anti-body heavy chain.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Immunohistology - Frozen
1:50 -
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA,please enquire.
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