HCA122A detects mouse calcium/calmodulin-dependent protein kinase II Inhibitor beta (CaMK2N2), also known as calcium/calmodulin-dependent protein kinase II inhibitor 2, a potent inhibitor of CaM kinase II (CAMK2). CaMK II N beta interacts with both CAMK2 alpha and beta.
The peptide sequence used to generate this antibody is identical to Rat, Bovine (Bos taurus) and Short tailed Opossum (Monodelphis sp.) CaMK II N beta.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Rat
Expected from Sequence
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed inE. coli.This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunohistology - Frozen
1/50 -
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Mouse brain (Hippocampus). Tissue sections require incubation with antibody for 72hrs.