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HUMAN ANTI CaMK II INHIBITOR BETA

  • 产品编号:HCA122A      品牌:AbD Serotec       原厂货号:HCA122A
  • 产品分类:抗体 > 一抗 > 蛋白特异性一抗
  • 应用分类:
 
包装: 50 μg
运保温度: +4oC
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描述:

Specificity

HCA122A detects mouse calcium/calmodulin-dependent protein kinase II Inhibitor beta (CaMK2N2), also known as calcium/calmodulin-dependent protein kinase II inhibitor 2, a potent inhibitor of CaM kinase II (CAMK2). CaMK II N beta interacts with both CAMK2 alpha and beta.

The peptide sequence used to generate this antibody is identical to Rat, Bovine (Bos taurus) and Short tailed Opossum (Monodelphis sp.) CaMK II N beta.

Species Cross-Reactivity

Target Species Cross Reactivity
Bovine Expected from Sequence
Rat Expected from Sequence

Product Form

A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.

 

Storage

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

12 months from date of reconstitution.

 

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.

 

Application Name Yes No Not Determined Suggested Dilution
Immunohistology - Frozen       1/50 -
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice

Protocols and further information may be found in the HuCAL Technical Manual

Immunohistology

This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

Histology Positive Control Tissue

Mouse brain (Hippocampus). Tissue sections require incubation with antibody for 72hrs.

 

GO Terms

UniProt

Health And Safety Information

Material Safety Datasheet Documentation #10041 available at: 
http://www.abdserotec.com/uploads/MSDS/10041.pdf

Acknowledgements

Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.

Licensed Use

For in vitro research purposes only, unless otherwise specified in writing by AbD Serotec.

Regulatory

For research purposes only

 


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