HCA116A647 recognises bovine FoxP3, a 48kD forkhead transcription factor family member, which was originally identified as the novel protein scurfin. FoxP3 functions as a DNA binding protein that represses transcription, and is reported to be involved in the regulation of T cell activation, differentiation and homeostasis.
FoxP3 is predominantly expressed within the nuclei of CD25 +CD4 + regulatory T cells (Tr). This population of FoxP3+CD25+CD4+ Tr cells plays an essential role in controlling autoimmunity.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed inE. coli.This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid conjugated to Alexa Fluor® 647.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Flow Cytometry(1)
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
(1)
Membrane permeabilization is required for this application. AbD Serotec recommends the use of 0.1% Triton X-100 for this purpose.
Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by AbD Serotec.
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