HCA115A recognises human desmin. Desmin belongs to the class III of intermediate filaments, constituting part of the cytoskeleton, and is the characteristic intermediate filament of all three types of muscle cells (skeletal, cardiac and smooth muscle). Desmin is a 52-kDa protein encoded by nine exons of a gene located at 2q35. Desmin forms cytoskeletal network across the muscle fibre bordering at the plasma and nuclear membrane and is particularly localized to the subplasmalemmal region and the Z-band. It is useful for identification of rhabdomyosarcomas and leiomyomas.
Product Form
A bivalent human recombinant Fab selected from the HuCAL ® phage display library, expressed in E.Coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. This antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA,please enquire.
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