HCA067A recognises human MEK 1, also known as mitogen-activated protein kinase kinase (MAPKK1). MEK 1 is a 43 kD dual specificity protein kinase which has the ability to phosphorylate both threonine and tyrosine residues. MEK1 acts downstream of Ras and Raf and is responsible for the phosphorylation of extracellular signal-regulated kinase (ERK) mitogen-activated protein kinase (MAPK). It is an integral component of the MAP kinase signalling cascade that regulates cell growth and differentiation, and may also play a role in brain synaptic plasticity.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed inE. coli.This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilised.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/100 - 1/250
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
HCA067A detects a band of approximately 43kDa in HCT-116 cell lysates.
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by AbD Serotec.
Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA,please enquire.
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